Table 2.
List of Primers Used for Real-Time Polymerase Chain Reaction
| Gene | Forward primer (5´–3´) | Reverse primer (5´–3´) |
|---|---|---|
| Accα | CTCCAGGACAGCACAGATCA | TGACTGCCGAAACATCTCTG |
| Acox1 | CAGGAAGAGCAAGGAAGTGG | CCTTTCTGGCTGATCCCATA |
| Adgre | CTTTGGCTATGGGCTTCCAGTC | GCAAGGAGGACAGAGTTTATCGTG |
| Atgl/pnpla2 | GAGCCCCGGGGTGGAACAAGAT | AAAAGGTGGTGGGCAGGAGTAAGG |
| Ccl2 | CAGGTCCCTGTCATGCTTCT | TCTGGACCCATTCCTTCTTG |
| Cxd68 | TGCGGCTCCCTGTGTGT | TCTTCCTCTGTTCCTTGGGCTAT |
| Cidea | CTCGGCTGTCTCAATGTCAA | GGGATGGCTGCTCTTCTGTA |
| Cox8β | GAACCATGAAGCCAACGACT | GCGAAGTTCACAGTGGTTCC |
| Cpt1α | CCAGGCTACAGTGGGACATT | GAACTTGCCCATGTCCTTGT |
| Dio2 | CAGTGTGGTGCACGTCTCCAA TC | TGAACCAAAGTTGACCACCAG |
| Fasn | TGGGTTCTAGCCAGCAGAGT | ACCACCAGAGACCGTTATGC |
| Hsl/lipe | GCCGGTGACGCTGAAAGTGGT | CGCGCAGATGGGAGCAAGAGGT |
| Lcad | CATTGGTGGGGACTTGCTCT | TGGCTATGGCACCGATACAC |
| Il1b | GCCCATCCTCTGTGACTCAT | AGGCCACAGGTATTTTGTCG |
| Il6 | ACAACCACGGCCTTCCCTACTT | CATTTCCACGATTTCCCAGAGA |
| Mcad | AGGTTTCAAGATCGCAATGG | CTCCTTGGTGCTCCACTAGC |
| p62 | AGAATGTGGGGGAGAGTGTG | TCGTCTCCTCCTGAGCAGTT |
| Pgc1α | ATGTGTCGCCTTCTTGCTCT | ATCTACTGCCTGGGGACCTT |
| Pparα | ATGCCAGTACTGCCGTTTTC | GGCCTTGACCTTGTTCATGT |
| Pparγ | TTTTCAAGGGTGCCAGTTTC | AATCCTTGGCCCTCTGAGAT |
| Prdm16 | CAGCACGGTGAAGCCATTC | GCGTGCATCCGCTTGTG |
| Ucp1 | AGGCTTCCAGTACCATTAGGT | CTGAGTGAGGCAAAGCTGATTT |
| β-actin | TGTTACCAACTGGGACGACA | GGGGTGTTGAAGGTCTCAAA |
| 36B4 | TAAAGACTGGAGACAAGGTG | GTGTACTCAGTCTCCACAGA |