Figure 2.

Myeloid-specific deletion of Notch1 alleviates LPS/D-GalN-induced liver inflammation. (A) The NICD expression was detected by Western blot assay in hepatic macrophages from the Notch1^FL/FL and Notch1^M-KO livers. (B) The representative gross appearance of the collected livers and histologic staining (H&E) of liver sections from Notch1^FL/FL and Notch1^M-KO mice after PBS or LPS/D-GalN injection (n = 5 mice/group). Scale bars, 100 μm. (C) Liver function in serum samples was evaluated by serum ALT and AST levels (IU/L) (n = 5 samples/group). (D) Immunohistochemistry staining and quantification of Ly6G⁺ neutrophils and F4/80⁺ macrophages in liver sections (n = 5 mice/group). Scale bars, 40 μm. (E) Quantitative reverse-transcriptase polymerase chain reaction–assisted detection of proinflammatory cytokines (Tnf-α, Il-6, and Il-1β), chemokines (Mcp-1 and Cxcl-1), and anti-inflammatory cytokines (Il-10 and Tgf-β) in liver tissues (n = 5 samples/group). (F) Kaplan-Meier survival curve comparing percent survival of Notch1^FL/FL and Notch1^M-KO mice treated with LPS (50 μg/kg) and D-GalN (700 mg/kg) (n = 9–11 mice/group). Data are presented as the mean ± standard deviation. ∗P < .05, ∗∗P < .01.