Figure 9.

Inhibition of YAP in macrophages attenuates LPS/D-GalN-induced liver injury. Notch1^FL/FL mice were injected by the tail vein with YAP-siRNA or NS control siRNA mixed with mannose-conjugated polymers at 6 hours before LPS/D-GalN treatment (n = 5 mice/group). (A) Representative H&E staining of liver sections. (B) Serum ALT and AST levels (U/L). (C, D) Immunohistochemical staining and quantification of Ly6G⁺ neutrophils and F4/80⁺ macrophages in liver sections. Scale bars, 40 μm. (E) Representative TUNEL-staining images and quantification of TUNEL⁺ cells in liver sections. Scale bars, 20 μm. (F) Quantitative reverse-transcriptase polymerase chain reaction–assisted detection of proinflammatory cytokines (Tnf-α, Il-6, and Il-1β), chemokines (Mcp-1 and Cxcl-1), and anti-inflammatory cytokines (Il-10 and Tgf-β) in liver tissues. Data are presented as the mean ± standard deviation. ∗P < .05, ∗∗P < .01.