Fig. 1.
Muscle characteristics with respect to treatment. (A) There was no significant difference in bodyweight between wildtype and D2.mdx mice. (B) Optimum muscle length (Lo) was not significantly different between wildtype and D2.mdx muscles. (C) Muscle PCSA was not significantly different between wildtype and D2.mdx muscles. (D) Decellularization treatment reduced the sample weight of diaphragm muscles but not solei. (E) Collagenase digestion treatment reduced the sample weight of diaphragm and soleus muscles. (F) Representative fluorescent images of raw, collagenase (coll-ase), and decellularized (decell) wildtype muscles. Scale bars are equal to 100 μm. Flat bars represent two-way ANOVA significant main effects by treatment: #p < 0.05, ##p < 0.01, ####p < 0.0001. Bracketed bars represent post-hoc Sidak test significant differences by treatment: #p < 0.05, ##p < 0.01, ###p < 0.001. DP = diaphragm, SOL = soleus, DCM = decellularized matrix, CD = collagenase digested, MyHC = myosin heavy chain, WGA = wheat germ agglutinin, Coll-ase = collagenase, Decell = decellularized.