Figure S5.

STAT6 activity can be therapeutically targeted and can resolve clinical disease severity. (A) Quantification of luciferase assay in HEK293 transfected cells pre-treated with ruxolitinib (10 μM, 1 h), tofacitinib (10 μM, 1 h), or dupilumab (10 nM, 1 h), before and after stimulation with IL-4 (0.02 ng/ml, 4 h). n = 4. One-way ANOVA and Tukey’s post-hoc test. *, P < 0.05; **, P < 0.01; ***, P < 0.001. (B) Eosinophil counts before and following initiation of treatment with dupilumab are presented. Dots in red corresponds to transcriptomic data from this patient presented in Fig. 6 C. (C) PCA comparing whole blood bulk RNAseq of P6 before treatment with dupilumab and four time points after treatment, alongside five healthy controls. (D) Heatmap signatures of differentially expressed genes comparing pre-treatment patient samples against five healthy controls. Genes are row normalized. (E) Key genes, previously described to be biomarkers for allergic disease (Lemonnier et al., 2020) in whole blood RNA are presented for the patient samples. Gray shaded area is the range for the expression of these genes in five healthy controls.