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. Author manuscript; available in PMC: 2023 Dec 23.
Published in final edited form as: J Vis Exp. 2022 Dec 23;(190):10.3791/64676. doi: 10.3791/64676

Figure 7: Determining organoid renewal capacity.

Figure 7:

Panel A: Experimental design to determine the organoid renewal capacity by subculture. The growing primary organoids (P0) are dissociated at various time points and made into single-cell suspensions. These cells are passaged to determine the OFR at day 7 in the subculture (P1). Panel B: Representative OFR data from passaged MTOs generated from 4NQO-untreated mice. Note that the OFR decreases as a function of time, reflecting decreased proliferative basaloid cells in the matured organoids, which contain more cells that have undergone post-mitotic terminal differentiation (see Figure 8). *** p < 0.001 (n = 6) OFR at day 7, day 11, and day 21 versus OFR at day 4.