Figure 2.

Evidence of tri‐trophic persistence of dsRNA. Agarose gel image showing the detection of dsGFP in Coccinella septempunctata ladybug larvae after consuming Myzus persicae aphids that had fed on Nicotiana benthamiana infiltrated with TRV2‐GFP. The expected amplicon size is 180 bp, which is consistent to the band size shown in the gel. PCR from cDNA samples of the following treatments are shown in each lane: Plants: plants that infiltrated with TRV2 empty vector (EV) or TRV2‐GFP vector (dsGFP); Aphids: aphids fed on EV and dsGFP VIGS plants; Ladybugs: ladybugs fed on aphids that were fed on EV and dsGFP VIGS plants. Each treatment was performed with three biological replicates (shown as three lanes), and this experiment was repeated twice. M: Promega 100 bp DNA ladder.