Figure 2.

Primary screen trends and down selection process (A) Modulators (N = 3147) alone show little inherent activity in either NF-κB or IRF transcription factors. (B) Modulation of transcription factor for NF-κB with LPS shown to act independently of IRF transcription factor modulation (R² = 0.0409). (C) Modulators demonstrate different trends across agonists: general enhancers (green), general inhibitors (red), or specialist activity (yellow). (D) Table summary of specialist modulators (active with only one agonist) and generalist modulators (active with 12–13 agonists). (E) Principle component analysis with IRF and NF-κB transcription factor data from all modulators (N = 3147) and high z-factor agonists (N = 8). (F) Compounds with minimal variability were removed by creating a circle centered on the origin with radius 1.75.