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. 2023 Jan 12;9(3):352–361. doi: 10.1021/acscentsci.2c01114

Figure 4.

Figure 4

Average weighted tryptophan fluorescence lifetime of different tubulin polymerization states as a function of AMCA concentration. (A) Free GTP tubulin compared to that of microtubules polymerized using GTP-tubulin, (B), microtubules polymerized using GTP-tubulin to that of those polymerized using GMPCPP-tubulin, (C) microtubules polymerized using GTP-tubulin in the presence and absence of etomidate and isoflurane. Dashed lines and shaded regions represent mean and standard deviation of tryptophan lifetimes in different tubulin polymerization states in the absence of AMCA. p-Values were calculated to determine significance of differences in average weighted lifetimes between GTP microtubules and other tubulin polymerization states (see Figure S10). Error bars represent standard deviation of experiments conducted n = 3 to n = 5 times.