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. 2023 Jan 12;3(2):143–156. doi: 10.1021/acsphyschemau.2c00061

Figure 1.

Figure 1

Illustration of three assays used in affinity-based single-molecule sensors. In a direct assay, the signal is generated by the analyte itself. In a sandwich assay, a tag or detection probe is used to provide a signal once an analyte is bound. In a competitive assay, a labeled competitor is detected whose interaction with the receptors is prevented in the presence of an analyte.