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(a) Experimental scheme for the detection of analytes (target antigen) by SiMREPS. (b) Single movie frame of a representative microscope FOV; the bright puncta represent single FPs bound at or near the coverslip surface. (c) Representative intensity versus time traces showing the distinct kinetic fingerprints of nonspecific binding (top) and repetitive binding to the analyte (bottom). Reproduced with permission from ref (39). Copyright 2020 American Chemical Society.
