Table 1.

Summary of the nine HKGs evaluated in this study.

Symbol	Name	Physiological Functions	RefSeq Accession No.	Bio-Rad Assay ID	Efficiency	r2	Amplicon Length (bp)
Actb	Beta-actin	Cell motility and cytoskeletal maintenance [43,44,45]	NM_031144	qRnoCID0056984	97	0.9987	74
B2m	Beta-2-microglobulin	Assembly and surface expression of MHC class I molecules [46]	N/A	qRnoCED0056999	95	0.9998	106
Gapdh	Glyceraldehyde-3-phosphate dehydrogenase	Glycolysis [47]; transcription activation; initiation of apoptosis [48,49]; vesicle trafficking [50]	NM_017008	qRnoCID0057018	96	0.9998	115
Hprt1	Hypoxanthine-guanine phosphoribosyltransferase	Purine nucleotide generation [51]	NM_012583	qRnoCED0057020	98	0.9989	79
Pgk1	Phosphoglycerate kinase 1	Phosphoprotein glycolysis [52]	NM_053291	qRnoCED0002588	98	0.9993	78
Rplp0	60S acidic ribosomal protein Stalk Subunit P0	Elongation step of protein synthesis [53,54]	NM_022402	qRnoCED0005242	100	1	97
Rplp2	Ribosomal Protein Lateral Stalk Subunit P2	Elongation step of protein synthesis [53,54]	N/A	qRnoCED0015635	89	0.9911	60
Tbp	TATA-box-binding protein	Activation of eukaryotic genes [55]	NM_001004198	qRnoCID0057007	95	0.9985	107
Tfrc	Transferrin receptor	Regulating stellate cell activation [56]	NM_022712	qRnoCID0003700	96	0.9998	66