Table 4.
List of antibodies used for spleen and PLN immunophenotyping.
| Target | Fluorophore | Species/Isotype | Clone | Use/100 µL | Company | |
|---|---|---|---|---|---|---|
| PANEL 1 | CD3 | PE | Hamster IgG | 500A2 | 1 | BD Biosciences |
| CD4 | APC | Rat IgG2a, κ | RM4-5 | 0.5 | BD Biosciences | |
| CD8 | V500 | Rat IgG2a, κ | 53-6.7 | 0.4 | BD Biosciences | |
| CD44 | BV786 | Rat IgG2b, κ | IM7 | 0.2 | BD Biosciences | |
| CD62L | APC-Cy7 | Rat IgG2a, κ | MEL-14 | 0.4 | BioLegend | |
| PD-1 | PE-Cy7 | Rat IgG2a, κ | 29F.1A12 | 2 | BioLegend | |
| CD25 | PerCP-Cy5.5 | Rat/IgG1, λ | PC61.5 | 1 | eBioscience | |
| FOXP3 | FITC | Rat IgG2a, κ | FJK-16s | 1.5 | eBioscience | |
| PANEL 2 | CD3 | PE | Hamster IgG | 500A2 | 1 | BD Biosciences |
| CD19 | V450 | Rat IgG2a, κ | 1D3 | 1 | BD Biosciences | |
| CD11c | PE-Cy7 | Hamster IgG1, λ2 | HL3 | 1 | BD Biosciences | |
| MHC-II | APC | Rat IgG2b, κ | AMS-32.1 | 1 | eBioscience |