Extended Data Fig. 5. No depletion of thymic epithelial cells in Mgl2^DTR-eGFP mice.

(a) Quantification of total numbers of CD301b⁺ cDC2 from BALB/c/BYJ (n=3), IL-4 KO (n=4) and IL-4Ra KO (n=4) mice. (b) Experimental strategy for selective depletion of CD301b expressing cells in Mgl2^DTR-eGFP mice. (c) Representative flow cytometric gating strategy of thymic epithelial cell (TEC) populations. Cells were MACS enriched as CD3⁻ CD4⁻ CD19⁻ TER119⁻. TECs were gated as CD45⁻ EpCAM⁺, then divided to cortical TECs (cTECs) and medullary TECs (mTECs) according to the expression of Ly51 and UEA, respectively. mTECs were then divided based on the Ly6d and MHC II expression to mTECs^Low (black), mTECs^High (blue), Pre-post-Aire (red), and Post-Aire (green). (d) Quantification of numbers of TEC populations (gated as in c) from diphtheria toxin (DTx) treated Mgl2^WT (gray dots) (n=5) or Mgl2^DTR-eGF (green dots) (n=5). Six to ten-week-old male and female mice were used. Small horizontal lines indicate mean, and error bars represent SD. Data are representative of at least 3 independent experiments (c) or are pooled from at least 2 independent experiments (a, d). ns=not significant, *P<0.05, ***P< 0.001. One-way ANOVA test with Tukey’s multiple comparisons test was used.