Extended Data Fig. 9. Frequencies of thymic T cell populations.

(a-c) Total CD4 T cells in mice with selective deficiencies. The administration of DTx into Mgl2^DTR-eGFP mice was done as in Figure 5d. (d and e) Frequency of CD5⁺ TCRβ⁺ cleaved caspase 3⁺ thymocytes among CCR7⁺ CD4 T cells in in mice with selective deficiencies (gated as in Extended Data Fig. 8). (f) Frequency of CD5⁺ TCR⁺ cleaved caspase 3⁺ thymocytes among DP T cells in Mgl2^WT (n=9) or Mgl2^DTR-eGFP (n=8). The administration of DTx into Mgl2^DTR-eGFP mice was done as in Figure 5d. (a-e) Aire^WT (n=5) or Aire^KO (n=6), Batf3^WT (n=9) or Batf3^KO (n=11) and Mgl2^WT (n=8) or Mgl2^DTR-eGFP (n=6) mice were used. (g) Frequency of CD25⁺FOXP3⁻ and CD25⁻FOXP3⁺ Treg cell progenitors (TRP) and (h) nascent CD25⁺ FOXP3⁺ CD73⁻ and recirculating CD25⁺ FOXP3⁺ CD73⁺ Treg cells in Mgl2^WT (n=9) or Mgl2^DTR-eGFP (n=8) mice following 9 days of diphtheria toxin treatment. The administration of DTx into Mgl2^DTR-eGFP mice was done as in Figure 5d. Each symbol represents an individual mouse. Six to twelve-week-old male and female mice were used. Small horizontal lines indicate the mean and error bars represent SD. ns=not significant, *P< 0.05, **P<0.01. Data are pooled from at least three independent experiments. Unpaired Mann-Whitney test was used.