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. Author manuscript; available in PMC: 2023 Mar 24.
Published in final edited form as: Nat Immunol. 2022 May 30;23(7):1042–1051. doi: 10.1038/s41590-022-01218-x

Extended Data Fig. 1. Thymic APC Gating Strategy.

Extended Data Fig. 1.

Flow cytometric gating strategy for identifying B cells and dendritic cell subsets. B cells were identified by the expression of CD19 and B220 (blue; top, middle). Plasmacytoid DC (pDC) were identified by the expression of B220, CD11c, and PDCA-1 (CD317) (salmon; bottom, middle). Conventional dendritic cells were identified by the expression of MHC II and CD11c (top, middle right). cDC1 were identified by the expression of XCR1 (magenta) and cDC2 were identified by the expression of SIRPα (teal; top, right). CD301b expression is shown on cDC1 (green; right) and cDC2 green; (green; bottom).