FIG 2.
Inhibition of methyltransferases with DZNep treatment on CDKN2A/B induction by TGFβ in RMS cells. (A) Quantitative analysis of Smad7 mRNA expression in tested RMS cell lines treated with vehicle or TGFβ for 72 h. (B–C) The effect of DZNep treatment on further ARF and INK4B induction by TGFβ in RD cells was determined by (B) protein levels through Western blots and (C) mRNA expression through qRT-PCR. (D) Quantitative analysis of mRNA expression of the indicated genes in tested RMS cell lines preincubated with DZNep following with TGFβ induction. For quantitative mRNA expression in panels C and D, gene expression of the indicated genes are normalized to the “H2O-V” group. V, Vehicle; T, TGFβ. Error bars: ±SEM. §P < 0.05, T vs. V; *P < 0.05, greater than “H2O-V”; #P < 0.05, greater than “DZNep-V”.