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. 2023 Mar 21;15(1):2193115. doi: 10.1080/19490976.2023.2193115

Figure 2.

Figure 2.

AIEC infection induced intestinal fibrosis and inhibited let-7b expression. (a) Quantification of AIEC strain LF82 (CFU/g) cultured from feces of WT and IL-10−/− mice with AIEC/K12 strain infection at day 10, day 20, day 30. (b) Body weight of the mice measured every two days and expressed as a percentage of the initial weight. (c) H&E and Masson’s trichrome staining of colon sections from AIEC/K12-infected IL-10−/−mice and from uninfected controls. Black triangles indicate submucosal edema and inflammatory cellular infiltrates. Grey triangles indicate collagen deposition in the colonic submucosa and mucosa. Scale bar, 200 μm. (d) Colon pathology scored expressed as an average of five views per section. (e) Quantification of intestinal fibrosis expressed as the percentage of collagen deposition in colon samples using ImageJ software. (f) The mRNA levels of TGFβ1, Col1a1, α-SMA and fibronectin in the colon determined by qPCR. Data were normalized to mouse 36B4 RNA. (g) The miRNA levels of let-7b analyzed by qPCR. (h) Correlation between the levels of let-7b and degrees of intestinal fibrosis represented by mRNA levels of TGFβ1 and Col1a1. Spearman’s test was used for correlation analysis. Data are expressed as the means ± SEM, n = 5 in each group. *P<.05, **P<.01, ***P<.001.