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. 2023 Mar 21;15(1):2193115. doi: 10.1080/19490976.2023.2193115

Figure 3.

Figure 3.

Upregulation of let-7b ameliorated intestinal fibrosis and inflammation. (a) The miRNA levels of let-7b in colon tissue in different groups measured by qPCR. (b&c) Body weight expressed as a percentage of initial weight. (d) H&E staining of colon sections and colon pathology scores and (e) Representative images and quantification of Masson’s trichrome staining of colon sections from IL-10−/− mice after let-7b agomir/antagomir treatment with/without AIEC infection. Scale bar, 200 μm. (f) The mRNA levels of TGF-β1, Col1a1, α-SMA and fibronectin expression in the colon determined by qPCR. The data were normalized to mouse 36B4 RNA and expressed as fold-change relative to the IL-10−/− mice control group. Let-7b ON: upregulation of let-7b with let-7b-agomir; let-7b OFF: inhibition of let-7b with let-7b-antagomir. Data are expressed as the means ± SEM, n = 5 in each group. *P<.05, **P<.01, ***P<.001.