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. 2023 Mar 21;15(1):2193115. doi: 10.1080/19490976.2023.2193115

Figure 7.

Figure 7.

AIEC regulates Mø polarization by modulating IECs-derived exosomal let-7b. (a) The miRNA level of let-7b in RAW264.7 and CT26 with/without AIEC infection measured by qPCR. U6 was used as an internal control. (b) The original miRNA levels of let-7b in RAW264.7 and CT26. (c) The changes of let-7b levels in RAW264.7 after co-cultured with non-infected and AIEC-infected CT26. (d) The red fluorescence of the Cy3-labeled let-7b mimic transferred from CT26 to RAW264.7 detected by confocal microscopy. Scale bar, 10 μm. (e) Electron microscopy analysis of exosomes secreted by CT26. Scale bar, 100 nm. (f) Particle size of the vesicles secreted from CT26 measured by ZetaView analysis. (g) Exosome-associated protein markers HSP70, CD63, and CD9 measured by western blot analysis. (h) The red fluorescence of Dil-labeled CT26-derived exosomes detected by confocal microscopy. Scale bar, 50 μm. (i) The miRNA levels of let-7b in RAW264.7 co-cultured with CT26 pretreated with/without the exosome secretion inhibitor GW4689 (20 μM). Data are expressed as the means ± SEM. All the experiments repeated 3 times. *P<.05, **P<.01, ***P<.001.