Figure 1.

Translational repression assay. A) in the absence of the RBP, translation of the reporter protein can take place. B) in the presences of the RBP-sfGFP fusion construct, translation is blocked. C) Schematic representation of the modified TagBFP mRNA reporter used as starting point for assay development, schematic representation of the SRSF1 domain structure, and the used SRSF1 protein construct GFP-SRSF1. D) Repression of TagBFP observed upon increasing expression of GFP-SRSF1 using a reporter with consensus sequence for SRSF1 (S1) or without (S0). The repression ratio is calculated by dividing the highest with the lowest TagBFP production rates.