Table 1.
Different reporter constructs used for translational repression assays with SRSF1.
| Con-struct | RBP binding sequence | Linker | Repression ratio | Basal TagBFP production rate (1/min) |
|---|---|---|---|---|
| S1 | AGAAGAAC | - | 3.3 ± 0.3 | 211.5 ± 23.1 |
| S2 | AGAAGAACAGAAGAAC | - | 9.0 ± 2.2 | 260.4 ± 57.3 |
| S3 | AGAAGAACAGAAGAACAGAAGAAC | - | 15.1 ± 2.2 | 280.1 ± 15.2 |
| S4 | AGAAGAAC | AUA | 4.8 ± 1.0 | 194.5 ± 33.1 |
| S5 | AGAAGAACAGAAGAAC | AUA | 12.5 ± 1.0 | 314.4 ± 14.8 |
| S6 | AGAAGAACAGAAGAACAGAAGAAC | AUA | 17.0 ± 0.4 | 301.5 ± 12.3 |
| S7 | AGAAGUACAGAAGAACAGAAGAAC | AUA | 14.2 ± 2.1 | 321.0 ± 20.2 |
| S8 | AGAAGAACAGAAGUACAGAAGAAC | AUA | 16.1 ± 4.0 | 305.3 ± 20.9 |
| S9 | AGAAGAACAGAAGAACAGAAGUAC | AUA | 11.8 ± 1.3 | 316.7 ± 18.8 |
| S10 | AGAAGUACAGAAGUACAGAAGUAC | AUA | 3.2 ± 0.4 | 219.4 ± 6.0 |
| S0 | - | - | 1.2 ± 0.0 | 217.5 ± 30.2 |
| S6–4 | AGAAGAACAGAAGAACAGAAGAAC | (AU)2 | 14.5 ± 1.6 | 283.3 ± 22.5 |
| S6–5 | AGAAGAACAGAAGAACAGAAGAAC | (AU)2A | 13.5 ± 1.1 | 276.2 ± 20.8 |
| S6–6 | AGAAGAACAGAAGAACAGAAGAAC | (AU)3 | 11.9 ± 2.3 | 294.1 ± 17.7 |
| S6–7 | AGAAGAACAGAAGAACAGAAGAAC | (AU)3A | 11.1 ± 2.7 | 265.0 ± 18.5 |
| S6–8 | AGAAGAACAGAAGAACAGAAGAAC | (AU)4 | 9.2 ± 1.6 | 256.5 ± 16.1 |
| S6–9 | AGAAGAACAGAAGAACAGAAGAAC | (AU)4A | 8.4 ± 3.1 | 201.5 ± 13.1 |
| S6–10 | AGAAGAACAGAAGAACAGAAGAAC | (AU)5 | 6.5 ± 0.8 | 155.4 ± 9.9 |