Table 3.

Affinities of SRSF1 and hnRNP A2/B1 for representative RNA sequences measured by fluorescence polarization.

RNA	Protein	KD (nM)	Hill slope
FAM-S1	SRSF1	>25000	-
FAM-S1ext	SRSF1	92.3 ± 44.1	1.33 ± 0.32
FAM-S2	SRSF1	56.1 ± 17.5	0.62 ± 0.30
FAM-S3	SRSF1	42.5 ± 33.9	0.53 ± 0.11
FAM-H1	hnRNP A2/B1	51.7 ± 9.0	0.75 ± 0.09
FAM-H2	hnRNP A2/B1	134.8 ± 9.0	0.66 ± 0.05
FAM-H3	hnRNP A2/B1	24.0 ± 8.1	0.52 ± 0.04