Figure 2.

Capsaicin and GPCR agonists induce visceral hypersensitivity in SPF mice but not in germ-free mice. (a) Representative traces of VMR to CRD at 300 μL in conscious SPF and germ-free (GF) mice in response to vehicle, capsaicin (30 μg) or GPCR agonists (30 μg). (b) AUC of the VMR after vehicle, capsaicin or GPCR agonists administered intracolonically (white circle: vehicle; light gray circle: capsaicin; dark gray circle: GPCR agonists). Data are represented as scatter dot plot with means (b). Statistical analysis was performed using Kruskal-Wallis followed by Dunn’s post hoc test.