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. 2023 Mar 20;15(1):2188874. doi: 10.1080/19490976.2023.2188874

Figure 3.

Figure 3.

GPCR agonists and capsaicin pre-treatment induce greater responses to CRD in germ-free female mice. (a) VMR of SPF (n = 29) and GF (n = 12) mice after intracolonic administration of capsaicin or GPCR agonists at distensions of 100 μL, 200 μL and 300 μL. VMR is expressed as % of baseline (white bar: SPF; gray bar: GF). (b) AUC of the VMR after intracolonic administration of capsaicin or GPCR agonists in SPF and GF (white circle: SPF; gray circle: GF). (c) VMR of SPF female (n = 12) and male (n = 17) and GF female and male (each n = 6) after administration of capsaicin. (d) AUC of the VMR after capsaicin intracolonic administration in SPF and GF female and male. (e) VMR of SPF female (n = 12) and male (n = 11) and GF female and male (both n = 6) after intracolonic administration of GPCR agonists. (f) AUC of the VMR after administration of GPCR agonists in SPF and GF. White bar/circle: SPF; gray bar/circle: GF. Data are represented as means±sem (a) (c) (e), scatter dot plot with means (b) (d) (f). Statistical analysis was performed using 2-way ANOVA followed by šidak’s multiple comparisons test (a) (c) (e) and Mann-Whitney t-test (b) (d) (f).