Table 1.

Tick pathogen detection assays

Tick type	Detection	Multiplexing	Target gene	Probe label dye
Hard ticks	DNA QC internal control Borrelia spp. Ixodes pacificus	Hard tick Triplex 1	16S mtDNA 16S CO1	FAM HEX Cy5
Ixodes ticks	Borrelia burgdorferi Borrelia miyamotoi Borrelia mayonii	Ixodes Triplex 1	ospA glpQ ospC	FAM HEX Cy5
Ixodes ticks	Babesia microti Anaplasma phagocytophilum Ehrlichia muris-like agent	Ixodes Triplex 2	Tubulin MSP-2 P13	FAM HEX Cy5
Non-Ixodes ticks	Ehrlichia chaffeensis Borrelia lonestari Ehrlichia ewingii	Non-Ixodes Triplex 1	dsb glpQ dsb	FAM HEX Cy5
Non-Ixodes ticks	Francisella tularensis Rickettsia rickettsii Anaplasma phagocytophilum	Non-Ixodes Triplex 2	ISFtu2 RRi6 MSP-2	FAM HEX Cy5