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. Author manuscript; available in PMC: 2023 Aug 7.
Published in final edited form as: Sci Signal. 2023 Feb 7;16(771):eabn8372. doi: 10.1126/scisignal.abn8372

Fig. 4. USP47 interacts with XIAP and TLE3.

Fig. 4.

(A) HEK293 cells were transfected with plasmids encoding Myc-XIAP and HA-USP47as indicated. HA immunoprecipitates (IP) and whole-cell lysates (WCL) were immunoblotted for HA and Myc. Arrowhead indicates autoubiquitylated Myc-XIAP. GAPDH is a loading control. (B) HEK293 cells were transfected with plasmids encoding Myc-XIAP and HA-TLE3 as indicated, and cell extracts were immunoprecipitated with an antibody recognizing endogenous USP47 or with IgG (negative control). Co-immunoprecipitated Myc-XIAP and HA-TLE3 were detected by immunoblotting for Myc and HA, respectively. Arrowhead indicates nonspecific band. (C) HEK293 cells were incubated in the presence or absence of purified recombinant Wnt3a, and cell extracts were immunoprecipitated with a USP47-specific antibody or IgG (negative control). Co-immunoprecipitated, endogenous XIAP and TLE3 were detected by immunoblotting. (D) HEK293 cells were transfected with the indicated expression constructs, and Myc-XIAP was immunoprecipitated. Co-immunoprecipitated V5-USP47 and HA-TLE3 were detected by immunoblotting for V5 and HA, respectively. Asterisk marks proteolytic fragment of USP47 occasionally observed during the immunoprecipitation step. All immunoblots are representative of at least three independent experiments.