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. Author manuscript; available in PMC: 2023 Aug 7.
Published in final edited form as: Sci Signal. 2023 Feb 7;16(771):eabn8372. doi: 10.1126/scisignal.abn8372

Fig. 5. USP47 and XIAP do not affect one another’s stability.

Fig. 5.

(A) HEK293 cells were transfected with non-targeting (NT) control or two independent USP47 siRNAs (USP47 #1 and #2). Whole-cell lysate was collected and immunoblotted for endogenous USP47, TLE3, and XIAP. GAPDH is loading control. (B) HEK293 cells were transfected as indicated with vector control (Con) or Myc-XIAP and treated with purified recombinant Wnt3a prior to immunoblotting for USP47 and XIAP. (C) HEK293 cells transfected with NT control or two independent XIAP siRNAs (XIAP #1 and #2) were incubated in the presence or absence of Wnt3a and cell extracts were immunoblotted for USP47 and XIAP. (D) In vitro–translated USP47 was incubated in an in vitro ubiquitylation assay with recombinant proteins as indicated and USP47 was detected by immunoblotting. All immunoblots are representative of at least three independent experiments.