Extended Data Fig. 2. Optogenetic silencing of vIRtPV neurons also impaired whisking.
a, A schematic of vIRt PV GtACR2 silencing experiment. b, Representative image of GtACR2 expression and the optic fiber track. c, Optic fiber tip placements over vIRt from all AAV-Flex-GtACR2-GFP injected animals. d, Experimental setup for vibrissa tracking. One second running triggers 1 second continuous 473nm laser stimulation. e, Example vibrissa trace during laser-OFF and -ON period. f, Quantification of whisking amplitude (Laser-OFF, 15.6 ± 0.7° vs. Laser-ON, 10.2 ± 1.1°, n = 5, p = 0.0312, Wilcoxon signed-rank test). g, Power spectrum analysis of whisking frequency in Laser-OFF and -ON period. Data are mean ± s.e.m. * P ≤ 0.05. Brain sections were counterstained with Neurotrace Blue (b).