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. 2023 Mar 14;19(3):e1010665. doi: 10.1371/journal.pgen.1010665

Fig 4. Both, strong inactivation of RIS as well as RIS::unc-58gf(strong) prevent sleep behavior during L1 arrest.

Fig 4

A-F) Ion channel expression in RIS controls calcium activity and sleep behavior. Mobility quiescence bouts (grey shade) correlate with RIS transients. Movement speed was calculated by tracking the position of the head neuron RIS. A) RIS::twk-18gf and B) RIS::egl-23gf(strong) larvae do not have detectable mobility quiescence bouts or RIS transients. C) RIS::egl-23gf(weak) has reduced mobility quiescence bouts. D) Wild-type RIS activation transients and immobility bouts. E) RIS::unc-58gf(weak) spends more time in mobility quiescence bouts. F) RIS::unc-58gf(strong) has no detectable mobility quiescence bouts and no RIS transients but a high constant activity. G) RIS activation and immobility are correlated only for the strains that also show sleeping behavior. Alignment of RIS activity and speed to mobility quiescence bout onset. **p<0.01, ***p<0.001, Wilcoxon signed rank test. H) Mobility quiescence is a function of RIS activity levels n.s. p>0.05, **p<0.001, ***p<0.001, Welch test with FDR correction for multiple testing. I) Long-term sleep fraction for RIS activity strains from day 1 to day 12 of starvation.