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. 2023 Mar 14;19(3):e1010665. doi: 10.1371/journal.pgen.1010665

Fig 5. RIS::unc-58gf impairs sleep following cellular stress and during the L1 molt in developing larvae.

Fig 5

A-F) Sleep fraction of worms of different RIS activity strains during the heat shock experiment. Animals were recorded continuously throughout the experiment. First, a 1h baseline was recorded at 22°C, then a 20 min heat shock period of 37°C (in red shade) was applied, after the heat shock, the temperature was lowered to at 22°C again to monitor the response to the heat shock. G) Quantification of time spent in sleep 20 min after the heat shock. Wild-type worms sleep on average 38.3%. All other RIS manipulations reduced quiescence. RIS::unc-58gf(strong) only sleeps on average 6.5% of the time. **p<0.001, ***p<0.001, Welch test with FDR correction for multiple testing. H) RIS::unc-58gf(strong) reduces lethargus sleep compared to wild type. ***p<0.001, Welch test.