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. 2023 Mar 14;19(3):e1010665. doi: 10.1371/journal.pgen.1010665

Fig 8. RIS::unc-58gf(strong) causes increased muscle activity.

Fig 8

A) We calculated the movement speed of the RIM head neuron to measure head movement speed. RIM speed was reduced in RIS::unc-58gf(strong). *p<0.05, **p<0.01, ***p < 0.001, Welch test for comparison between strains, Wilcoxon signed rank test for comparison between the same strain quiescent and mobile bouts. B) We calculated the movement speed of the PLM tail neuron to measure tail movement speed. PLM movement speed was not significantly changed in mobile bouts in RIS::unc-58gf(strong) and increased in detected quiescent bouts. *p<0.05, **p<0.01, ***p < 0.001, Welch test for comparison between strains, Wilcoxon signed rank test for comparison between the same strain quiescent and mobile bouts. C-E) Sample traces of muscle activity for RIS::twk-18gf and RIS::unc-58gf(strong). F) RIS::unc-58gf(strong) causes increased muscle activity in quiescence as well as mobility bouts. *p<0.05, **p<0.01, ***p < 0.001, Welch test for comparison between strains, Wilcoxon signed rank test for comparison between the same strain quiescent and mobility bouts. G) A sleep bout alignment reveals that RIS::unc-58gf(strong) has increased muscle activity. **p<0.01, ***p < 0.001, Wilcoxon signed rank test. H) RIS::twk-18gf and RIS::unc-58gf(strong) reduced phases of muscle inactivity. *p<0.05, Welch test.