Table 2.

Differentially expressed probes 3 and 7 days after IM PanAd3-RSV prime vaccination with significant statistical correlation to clinical/immunological responses measured at later time points

Vaccination	Number of genes correlated with PRNT 28 days post vaccination	Number of genes correlated with IFNγ 14 days post vaccination	Number of genes correlated with VnAb 7 days post vaccination	Number of genes correlated with ASC-G 7 days post vaccination	Number of genes correlated with ASC-A 7 days post vaccination
3 Days post PanAd3 priming	43	15	4	23	20
3 days post MVA boost (IM PanAd3 primed)	6	0	NA	0	0
3 days post MVA boost (IN PanAd3 primed)	32	20	NA	9	6
7 Days post PanAd3 prime	42	11	85	73	44
7 days post MVA boost (IM PanAd3 primed)	15	57	18	94	23
7 days post MVA boost (IN PanAd3 primed)	12	53	51	19	31

Data from volunteers in study groups one, two, three, and four combined (n = 42). The significant correlations (Pearsons P-value 0.05) between changes in gene expression from the 634 of differentially expressed probes 3 days after IM PanAd3-RSV prime and the 782 and 94 probes, respectively differentially expressed after boost vaccination with MVA-RSV after either intramuscular or intranasal priming and log₂-transformed fold-change in serum anti-PanAd3 vector neutralising antibody 4-weeks after vaccination (VnAb), log₂-transformed fold-change in serum RSV-neutralising antibody titre 28 days after vaccination (PRNT), log₂-transformed anti-F IgG and IgA antibody secreting cell count 7 days after vaccination (ASC-G, ASC-A), and the log₂-transformed fold-change in IFNγ count 14 days after prime.