Fig. 3. The transcriptome of DPT is unique relative to CD4 and CD8 T cells.

(A) Venn diagram of the number of independent and shared differentially expressed genes among the three comparisons indicated. (B) Unsupervised hierarchical clustering of the RNA-seq samples using the computed Pearson correlation for each pair of samples and the Euclidean distances of the correlation distances determined. (C and D) Volcano plots showing the number of differentially expressed genes in the DPT:CD4 (C) and DPT:CD8 (D) comparisons using a cutoff of a ≥1 log expression difference and a P value of 0.01. (E) List of the 63 genes differentially expressed in both the DPT:CD4 and DPT:CD8 comparisons, with red indicating increased expression in DPTs relative to CD4/CD8 T cells and blue indicating decreased expression.