Skip to main content
. 2023 Mar 24;9(12):eadf0567. doi: 10.1126/sciadv.adf0567

Fig. 5. DPTs develop from CD8 T cells after antigenic stimulation.

Fig. 5.

(A) Unsupervised tSNE clustering of human T cells concatenated from five xeno-GVHD mice. (B and C) Isolated CD3, CD4, and CD8 PB-Tc from a healthy donor were transplanted into mice. Dot plots show the percentage of DPTs in mouse blood at 3 weeks after transplant (B) with the percentage of DPT quantified in (C). (D) Five mice transplanted with PB-MNC were euthanized, and the human cells were isolated before flow sorting of CD4 and CD8 T cells. (E and F) Dot plots and bar graph of the percentage of DPTs in the blood of mice retransplanted with flow-sorted CD4 and CD8 T cells. (G to I) CD8 T cells were cocultured for 5 days with mouse MNC with or without tacrolimus and then analyzed for DPTs (H) and IFN-γ (I). Parametric t test was used to determine significance. ***P < 0.001.