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. 2023 Mar 24;14:243–257. doi: 10.18632/oncotarget.28390

Figure 5. NSL-YHJ-2-27 suppresses cancer cell migration and invasion.

Figure 5

(A) Confluent monolayers of cancer cells separated by a “wound” generated using cell culture inserts (ibidi) were treated with the indicated concentrations of NSL-YHJ-2-27 and closure of the wounds was monitored, and images captured at 0 and 24 h after treatment using a Nikon Ti Eclipse microscope at 4X magnification. The number of cells that migrated into the wounds were counted. (B) A549 cells were plated onto the inserts of 24-well Matrigel invasion chambers after treatment and incubated for 24 h as indicated in the Methods. Cells that invaded from the top chamber of inserts through Matrigel were trapped on the membrane in the lower chamber of the inserts. These invading cells were fixed and then stained with 1% crystal violet. Bright field images were obtained using Nikon Eclipse microscope at 4X magnification. The results are the means of three independents experiments. Statistical significance (** p < 0.01, and *** p < 0.001) was determined using 1-way ANOVA with post hoc Dunnett’s tests.