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. 2023 Mar 24;13:4847. doi: 10.1038/s41598-023-31842-y

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Hair growth-promoting drug testing on hair follicloids. (a) Microscope images of hair follicloids cultured with/without minoxidil (MNX) for 10 days. Hair follicloids were permeabilized and observed using a stereomicroscope. (b) The length of sprouting structures with/without MNX. The graph shows the ratio of the length on days six, eight, and ten compared to that on day four. Numerical variables were statistically evaluated using Student’s t-test; * indicates p < 0.05. (c) Relative expression of hair growth-associated genes. GAPDH was used as a reference gene to normalize expression. Error bars represent the standard error of the mean calculated from three experiments for each condition. Numerical variables were statistically evaluated using Student’s t-test; * indicates p < 0.05.