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. 2023 Mar 24;13:4847. doi: 10.1038/s41598-023-31842-y

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Preparation of hair follicloids using patient hair follicle-derived cells. (a) Preparation of hair follicle stem cells (HFSCs) and dermal papilla (DP) cells from hair follicles of patients with androgenic alopecia. Dissociated HFSCs and DP tissues were seeded on the dishes for primary culture. Proliferated HFSCs and DP cells were suspended in culture media containing Matrigel and cultured in a 96-well plate. (b) Native human hair follicles. Microscope and immunostaining images were taken before cell dissociation. (c) DP cell culture. Isolated DPs adhered to and proliferated on the culture dishes. (d) Expression of stem cell markers in HFSC and DP cells. Primary cultures containing HFSCs and DP cells were visualized through the immunohistochemical staining of versican and CD200. (e) Time-course of sprouting of hair follicloids.