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. 2023 Mar 17;2023:7612566. doi: 10.1155/2023/7612566

Figure 1.

Figure 1

Schematic diagram of the construction of a large-capacity phage antibody display library: ①: mRNA isolation from hen B-cells; ②: RT-PCR; ③: scFv assembly by overlapping PCR; ④: ligation; ⑤: electrotransformation; ⑥: phage packaging.