Figure 1.
Schematic diagram of the construction of a large-capacity phage antibody display library: ①: mRNA isolation from hen B-cells; ②: RT-PCR; ③: scFv assembly by overlapping PCR; ④: ligation; ⑤: electrotransformation; ⑥: phage packaging.
Schematic diagram of the construction of a large-capacity phage antibody display library: ①: mRNA isolation from hen B-cells; ②: RT-PCR; ③: scFv assembly by overlapping PCR; ④: ligation; ⑤: electrotransformation; ⑥: phage packaging.