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. 2023 Mar 17;2023:7612566. doi: 10.1155/2023/7612566

Figure 3.

Figure 3

Summary of the construction of hTK1-IgY-scFv phage antibody libraries from immunized hens: (a) agarose gel electrophoresis detected the isolated RNA; (b) the antibody VL fragment was obtained by RT-PCR; (c) the VH fragment of the antibody was obtained by RT-PCR; (d) the scFv gene fragment was obtained by overlap PCR; (e) colony PCR was performed on 50 random clones in a total of 340 clones. A negative clone was detected (#3 in red box). Lane Mr. DNA marker: DNA 2000, 1000, 750, 500, 250, and 100 bp.