Table 1.
The primers used in the construction of the phage display hen scFv library.
| Primers | Sequences (5′-3′) | PCR steps |
|---|---|---|
| VH-B1 | GTCATCAGGCCCAGCCGGCCATGGCCGCCGTGACGTTGGACGAGTCCGG | VH-1st |
| VH-F1 | GCCTGAGCCGCCACCACCGGAGGAGACGATGACTTCGGT | |
| VL-B1-1 | AGTGGTGGAGGAGGATCCCAGGCAGCGCTGACTCAGCC | VL-1st |
| VL-B1-2 | AGTGGTGGAGGAGGATCCCAGGCAGCACTGACTCAGCC | |
| VL-B1-3 | AGTGGTGGAGGAGGATCCCAGGCAGCGCTGACTCTGCC | |
| VL-B1-4 | AGTGGTGGAGGAGGATCCCAGGCAGCAGTGACTCAGCC | |
| VL-B1-5 | AGTGGTGGAGGAGGATCCCAGGCAGCACCGACTCAGCT | |
| VL-F1 | GTTACTGGTTTGCCTGCGGCCGCTAGGACGGTCAGGGTTGTCCC | |
| VH-B2 | CTTAGAGTCATCAGGCCCAGCCGGCCATGGCC | VH-2nd |
| VH-F2 | TCCTCCACCACTTCCGCCGCCGCCTGAGCCGCCACCACC | |
| VL-B2 | CGGCTCAGGCGGCGGCGGAAGTGGTGGAGGAGGATCC | VL-2nd |
| VL-F2 | CGATGGTTAGCGTTACTGGTTTGCCTGC | |
| VH-B3 | CTTAGAGTCATCAGGCCCAGCCGGCC | scFv-3rd |
| VL-F3 | CGATGGTTAGCGTTACTGGTTTG |