(b) HTK1 calibrators were used to evaluate the stability and accuracy in four batches of hTK1-IgY-rmAb#5 using the semiautomatic ECL dot blot assay with the BSA platform and the automatic chemiluminescence analyser with the sandwich-BSA platform. Pearson correlation score (r > 99.9, data not shown)
hTK1 calibrator (pM) | Mean ± SD (OD value) | CV% |
---|---|---|
ECL dot blot | ||
2.2 | 11,029 ± 860.2 | 7.80 |
6.6 | 26,636 ± 2,087.9 | 7.92 |
20 | 99,985 ± 4,950.3 | 4.95 |
Automatic chemiluminescence | ||
1 | 753,128 ± 18,828.2 | 2.50 |
2 | 1,481,435 ± 16,295.8 | 1.10 |
5 | 3,638,642 ± 40,025.1 | 1.10 |
10 | 7,420,457 ± 51,943.2 | 0.70 |
20 | 14,854,175 ± 65,358.4 | 0.44 |