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. 2023 Mar 17;2023:7612566. doi: 10.1155/2023/7612566

(b) HTK1 calibrators were used to evaluate the stability and accuracy in four batches of hTK1-IgY-rmAb#5 using the semiautomatic ECL dot blot assay with the BSA platform and the automatic chemiluminescence analyser with the sandwich-BSA platform. Pearson correlation score (r > 99.9, data not shown)

hTK1 calibrator (pM) Mean ± SD (OD value) CV%
ECL dot blot
 2.2 11,029 ± 860.2 7.80
 6.6 26,636 ± 2,087.9 7.92
 20 99,985 ± 4,950.3 4.95
Automatic chemiluminescence
 1 753,128 ± 18,828.2 2.50
 2 1,481,435 ± 16,295.8 1.10
 5 3,638,642 ± 40,025.1 1.10
 10 7,420,457 ± 51,943.2 0.70
 20 14,854,175 ± 65,358.4 0.44