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. Author manuscript; available in PMC: 2024 Apr 1.
Published in final edited form as: Gastroenterology. 2023 Jan 10;164(4):593–609.e13. doi: 10.1053/j.gastro.2023.01.002

Figure 4.

Figure 4.

Myeloid cell infiltration is increased in DSS-induced colitis. (A) Representative gating of CD4+ T cells, B cells, Cd11b+ myeloid cells, neutrophils, macrophages, and Ly6Chigh/int/low subset of macrophages from flow cytometric analysis of C57BL/6J wild-type mice treated with various colitis-inducing agents. Citro, C rodentium; Doxo, doxorubicin; Oxa, oxazolone. One representative from each experimental group is shown. (B) Quantification of each immune cell subset as defined in panel A identifying F4/80+Ly6Chigh macrophages and Ly6G+ neutrophils as myeloid cells that are increased in DSS-treated mice compared with other models of colitis. Data are expressed as the percentage of total white blood cells (n ≥ 5 per group). (C) Representative images of immunofluorescent staining of F4/80+ macrophages, CD4+ T cells, and CD45R+ B cells in the colonic tissues of mice treated with various colitis-inducing agents. Scale bar: 100 μm. Data in all bar graphs are presented as mean ± SEM. Asterisks above each bar indicate significant differences from the control group. *P < .05, **P < .01, ***P < .001, ****P < .0001.