Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Mar 24;9:32. doi: 10.1038/s41421-023-00533-3

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 1 — a Co-IP analysis of Flag-tagged KCTD7 and myc-tagged Cullin-3 in HEK293 cells. b Co-IP analysis of KCTD7 mutant constructs and Cullin-3-Myc in HEK293 cells. c Co-IP analysis of KCTD7 deletion constructs and Cullin-3-Myc in HEK293 cells. d Co-IP analyses of Flag-tagged KCTD7, myc-tagged Cullin-3, and HA-tagged Rbx1 in HEK293 cells. e Co-IP analyses of GFP-tagged KCTD7 and Flag-tagged calpain 1, calpain 2, and CAPNS1 in HEK293 cells. f Confocal microscopy of HeLa cells showing KCTD7-GFP co-distribute with calpain 1-Flag, calpain 2-Flag, and CAPNS1-Flag. Trace outline is used for line-scan (white dashed line) analysis of relative fluorescence intensity of indicated protein signals. Scale bar, 10 μm. g Co-IP analyses of myc-tagged Cullin-3 with Flag-tagged calpain 1, calpain 2 and CAPNS1 in HEK293 cells. h Co-IP analyses of Flag-tagged KCTD7 mutant constructs with endogenous calpain 1, calpain 2, and CAPNS1 in HEK293 cells.