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. 2023 Mar 24;9:32. doi: 10.1038/s41421-023-00533-3

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a, b Ubiquitous expressing calpains were expressed in HEK293 cells and α-spectrin cleavage was evaluated by immunoblotting, n = 3. c In vivo ubiquitination assay for calpains performed by coexpressing with HA-Ub in HEK293 cells for 36 h. d Co-IP analysis of Flag-tagged calpains and myc-tagged Cullin-3 in HEK293 cells. e Co-IP analysis of Flag-tagged calpains and myc-tagged KCTD7 in HEK293 cells. f In vivo ubiquitination assay in HEK293 cells for calpains performed by expressing alone or in combination with Cullin-3. g In vivo ubiquitination assay in HEK293 cells for calpains performed by expressing alone or in combination with KCTD7.