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. 2023 Mar 24;14(3):208. doi: 10.1038/s41419-023-05741-z

Fig. 5. Exogenous LPA enhanced inflammatory response in vitro and vivo.

Fig. 5

A Effect of si-GPAT3 or si-N.C. on TG content in KCs stimulated with LPS (1 μg/ml) for 24 h (n = 3). B Bodipy 493/503 fluorescence visualized by fluorescence microscopy (scale bars represent 50 μm) (n = 3). C LPA concentrations in the supernatant of KCs after LPS (100 ng/ml 12 h) stimulated and with or without si-GPAT3 (n = 3). D The mRNA expression of IL-1β, NLRP3, IL-1α and IL-6 in KCs treated with LPA (30 μM 12 h) (n = 3). E, F The effects of exogenous LPA (30 μM 12 h) on the protein expression of inflammatory factors IL-1β, NLRP3, COX2, IL-1α and IL-6 in KCs (n = 3). G Experimental design of mice treated with LPA. H, I The contents of LDH, IL-6 and IL-1βin plasma of LPA treated with mice (n = 6). J, K Representative images and quantification of HE staining (Scale bars represent 100 μm) and CD68 IHC (Scale bars represent 200 μm) after LPA treatment in liver of mice (n = 3). Data represents mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001.