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. 2023 Mar 24;14:1635. doi: 10.1038/s41467-023-37237-x

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a General structural elements of SNACIP inducers in which the small molecule binding motif can be introduced either synthetically or posttranslationally. b Schematic view of the structure and working mechanisms of cRGT. c Structural elements of CysTMP (left) and Cys-cR10* (r: D-Arg, R: L-Arg) (right). d Two-step assembly of cRGT (IV) via EPL followed by disulfidization chemistry. e Representative size-exclusion chromatographic (SEC) analysis of 1 nmol EGFP, eDHFR or GBP-TMP (III) using Superdex 200 Increase 10/300 GL column at a flow rate of 0.4 ml·min^-1 revealed retention volumes (VR) of 16.2 ml, 16.7 ml and 18.4 ml, respectively. f SEC analysis of 1 nmol of EGFP/GBP-TMP/eDHFR ternary complex (solid line) which revealed a single peak at VR 14.1 ml while SEC analysis of a mixture of 1 nmol EGFP and 1 nmol eDHFR in the absence of GBP-TMP (dashed line) revealed no complex formation; same SEC instrumentation and parameters were applied as in e. g Denaturing SDS-PAGE analysis of EGFP, eDHFR and GBP-TMP used in e as well as the EGFP/GBP-TMP/eDHFR ternary complex corresponding to the VR 14.1 ml peak in f.