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. 2023 Mar 24;14:1635. doi: 10.1038/s41467-023-37237-x

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a Structural elements of cRTC (left) and schematic presentation of how cRTC “OFF”-regulates the key microtubule nucleation factor TPX2 (right). b Schematic view of the assembly of cRTC inducer (BCN: bicyclonornyne which reacts with azide via copper-free click reaction; Mal: maleimide which reacts with cysteine). c Live HepG2 cells coexpression of EGFP-CAAX (PM marker) and mScarlet-hTPX2 (hTPX2 marker) with or without (control) adding 10 μM cRTC (1.5 h) reveal the repositioning of hTPX2 to PM. d, e Line profile analysis of the cells shown in c. f Statistical PCC analysis between EGFP-CAAX and mScarlet-hTPX2 for cells in c (n = 20 cells for Control, n = 13 cells for cRTC); one-sided Student’s t-test was used; see Methods for description of box plots. Yellow scale bars: 5 μm.