Figure 5.

Histopathology and immunohistochemistry of TOV-112D original tumour. A. H&E-stained sections depicting multiple different regions of the original tumour. The tumour grows as glands (upper left) with focal sex cord differentiation (upper right). The tumor shows an abrupt transition from the glandular and corded region (asterisk *) to a broad undifferentiated region growing as solid sheets and nests (double asterisk **). B. Immunohistochemistry for SMARCA4/BRG1, SMARCA2/BRM, ER, PR, p53, and WT1 in the region showing loss of differentiation depicted in the lower panels in Part A. Images show loss of staining for SMARCA4/BRG1, SMARCA2/BRM, ER, and PR in the undifferentiated region and diffuse strong staining for p53 throughout the tumour, consistent with a missense mutation in the TP53 gene. No nuclear staining for WT1 is observed (only vascular staining, a positive internal control). H&E-stained images in Part A: upper panels at 100x magnification, lower left at 40x, lower right at 200x. Immunohistochemistry in Part B at 100x magnification.