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. 2023 Mar 24;24:116. doi: 10.1186/s12859-023-05226-y

Fig. 3.

Fig. 3

Decreased read support across homopolymer stretches. Points denote the most-evidenced base at each position across this region of BCRxV.VSVG with a 17mer homopolymer. Due to difficulty base calling the exact number of bases in homopolymer regions, many reads report fewer than 17 bases. This results in an apparent coverage drop off across the homopolymer. The horizontal dashed grey line at 10% of the max coverage represents the minimum base coverage required to add a base to the consensus. Note that for this visualization, indels were right-aligned to show coverage dropping from left to right